ELISA kit biological help antibody preparation
Ascites: After recombination of ascites, a small amount of sodium azide can be added to prevent bacterial growth. Ascites should be frozen. It is best to store monoclonal antibodies in small packages to avoid repeated freeze-thaw cycles. If it is to be stored for a long time, do not store at 4 ℃. Unlike serum, ascites contains protease, which will reduce antibody titer, so adding protease inhibitors can be an option.
Pure IgG: Do not dilute the antibody for later storage unless adding goat serum, BSA, etc. IgG will adhere to glass, plastic, etc. When the concentration of IgG is less than 0.1 mg / ml, it will be quickly absorbed and denatured, and lose its activity. And repeated freezing and thawing of pure IgG will definitely reduce its activity.
Serum: Serum is more durable than mAb. Add antibiotics can be stored at 4 ℃. The serum of the ELISA kit does not need to be added because it has a protease inhibitor. Cryopreservation is a better choice.
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