1. The newborn calf for blood supply must be the litter of healthy cattle and draw blood within 24 hours after delivery. During this period, breast milk should not be taken.
2. Bleeding from the carotid artery by aseptic operation, and separating the serum and the filter bacteria under aseptic conditions, the whole process is completed within 36 hours. Serum from the bacteria was stored in a 2 0 IC.
3. The serum should be used for fine shadow, mycoplasma culture and endotoxin measurement before use. It can be used under the condition of no pollution.
4. The total protein content of each batch of calf serum was determined and formulated into 10%, 20% and 25% respectively. The myeloma cells and hybridoma cells were cultured in the tomb and the HAT culture to observe the calf serum. Cytotoxicity of tumor cells and fused cells. If the cell growth is poor or death in the case of 20% concentration of calf serum, the batch of calf serum is not suitable for application.
How to freeze the serum will not damage the quality of the product:
1. After the serum is taken out of the freezer, it is first dissolved in a refrigerator at 2-8 ° C, and then completely dissolved at room temperature. However, it is necessary to pay attention to the fact that it is necessary to shake it evenly during the dissolution process.
2. The serum is frozen and found to have flocculent deposits. How to deal with it 3. There are many reasons for the presence of sediment in serum, but the most common cause is due to the degeneration of serum lipoproteins; and fibrin in serum After freezing, it is also present in serum and is one of the main causes of serum deposits. But these flocculent deposits do not affect the quality of the serum itself. If you want to remove these flocculent deposits, the serum can be dispensed into a sterile centrifuge tube and centrifuged slightly at 400g. The supernatant can then be filtered together in the incubation base. It is not recommended that you remove these flocculent deposits by filtration as it may clog your filter membrane.
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