Green tea beverage, as a daily drink, is rich in polyphenols, such as flavonoids and phenolic acids. Among them, flavonoids such as quercetin and kaempferol can be used as free radical scavengers, and have the effects of lowering blood pressure, anti-cancer and anti-oxidation. Chitosan is the second largest natural polymer after cellulose. It has certain health benefits and can effectively remove heavy metal ions and acid dyes.
The research group of medicinal chemical composition led by the researcher Yanping Ping, the Key Laboratory of Northwestern Characteristic Plant Resources Chemistry, Chinese Academy of Sciences, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, used magnetic carrier technology to design a new magnetic recovery chitosan solid-phase extraction model, combined with high efficiency The liquid chromatography-diode array detection method has successfully established a qualitative and quantitative analysis technology that detects the purification and enrichment of flavonoids in green tea beverages.
The advantages of this solid phase extraction mode are: chitosan as an adsorbent, directly extracts the flavonoids active substances in green tea beverages; after the extraction is completed, using ferric oxide superparamagnetic nanoparticles as a carrier, chitosan Recycled from green tea beverages. The new method for separation and analysis has a recovery range of 96.4% to 108.6%; a relative standard deviation range of 0.6% to 8.7%, and a correlation coefficient variation range of 0.9917 to 0.9988; when the signal-to-noise ratio is 3, the detection limit range is 5.4 to 16.8ng mL-1.
The magnetic recovery chitosan magnetic carrier technology solves the problems that the chitosan-modified iron oxide composite material cannot be used in an acidic medium, the extraction rate is low, and the chitosan is difficult to separate from the sample solution. The technology is fast, easy to operate, low in cost, and has no pollution to the environment.
A New Method for Solid Phase Extraction of Active Ingredients in Green Tea Beverages Discovered by Lanzhou Institute of Chemicals, Chinese Academy of Sciences
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