Preparation methods of common test solutions for analytical chemistry

The potassium hydroxide test solution made from ethanol can be obtained by using ethanol potassium hydroxide titration solution (0.5mol / L).
Take ethanol as the ammonia test solution and add concentrated ammonia solution to make 9 ~ 11g of NH3 per 100ml. This solution should be stored in a rubber stopper bottle.
4g silver nitrate test solution made of ethanol, dissolve silver nitrate, add 10ml of water to dissolve, add ethanol to make 100ml, then get. Use 2.5g mercury bromide in ethanol-made mercury bromide test solution, add 50ml of ethanol, and dissolve with slight heat. This solution should be placed in a glass stopper and kept in a dark place.
The iodine monochloride test solution is obtained by taking 0.14 g of potassium iodide and 90 mg of potassium iodate, adding 125 ml of water to dissolve, and then adding 125 ml of hydrochloric acid. This solution should be placed in a glass bottle, sealed and kept in a cool place.
N-Acetyl-L-Tyrosine ethyl ester test solution Take 24.0mg of N-Acetyl-L-Tyrosine ethyl ester, add 0.2ml of ethanol to dissolve, add phosphate buffer solution (take 0.067mol / L potassium dihydrogen phosphate Solution 38.9ml and 0.067mol / L disodium hydrogen phosphate solution 61.6ml, mixed, pH value 7.0ml 2ml, add indicator solution (take equal amount of 0.1% methyl red ethanol solution and 0.05% methylene blue ethanol solution , Mix) 1ml, diluted with water to 10ml, that is.
P-dimethylaminobenzaldehyde test solution prepared from ethanol Take 1 g of p-dimethylaminobenzaldehyde, add 9.0 ml of ethanol and 2.3 ml of hydrochloric acid to dissolve, and then add ethanol to 100 ml to obtain.
Sodium diethyldithiocarbamate test solution: Take 0.1 g of sodium diethyldithiocarbamate, dissolve it with 100 ml of water, and filter it.
Take 2g of m-dinitrobenzene in dinitrobenzene test solution, add ethanol to dissolve it into 100ml, then get.
Take dinitrobenzoic acid test solution 1g 3,5-dinitrobenzoic acid 1g, add ethanol to dissolve into 100ml, that is, get.
Take 2,4-dinitrophenylhydrazine 1.5g in dinitrophenylhydrazine test solution, add 20ml of sulfuric acid solution (1 → 2), dissolve, add water to make 100ml, filter, then you get.
Silver diethyldithiocarbamate test solution Take silver diethyldithiocarbamate 0.25g, add appropriate amount of chloroform and triethylamine 1.8ml, add chloroform to 100ml, stir to dissolve, let stand overnight, filter with cotton wool , That's it. This solution should be placed in a brown glass bottle, tightly closed, and stored in a cool place.
Take 1g of diphenylamine in the diphenylamine test solution, add 100ml of sulfuric acid to dissolve, then you get it.
Take diaminonaphthalene test solution 0.1g of 2,3-diaminonaphthalene and 0.5g of hydroxylamine hydrochloride, add 100ml of 0.1mol / L hydrochloric acid solution, heat to dissolve if necessary, let cool and filter. This solution should be freshly prepared before use and protected from light.
Dilute dinitrophenylhydrazine test solution, take 2,4-dinitrophenylhydrazine 0.15g, add 100ml of aldehyde-free ethanol containing 0.15ml of sulfuric acid to dissolve.
Take mercury dichloride test solution 6.5g mercury dichloride, add water to dissolve into 100ml, that is.
The dichloroindophenol sodium test solution is obtained by taking 0.1 g of 2,6-dichloroindophenol sodium, dissolving it with 100 ml of water, and filtering it.
Take 1g of dimethylglyoxime and add 100ml of ethanol to dissolve it.
Trinitrophenol test solution This solution is a saturated aqueous solution of trinitrophenol.
Lithium trinitrophenol test solution is obtained by taking 0.25g of lithium carbonate and 0.5g of trinitrophenol, adding 80ml of boiling water to dissolve, leaving to cool, and adding water to make 100ml. Take 9g of ferric chloride in the ferric chloride test solution, add water to dissolve it into 100ml, and you get it.
Trichloroacetic acid test solution: Take 6g of trichloroacetic acid, add 25ml of chloroform to dissolve, add 0.5ml of 30% hydrogen peroxide solution, shake well, then you get.
Take the appropriate amount of vanadium pentoxide in the test solution of vanadium pentoxide. After adding phosphoric acid, shake it vigorously for 2 hours to obtain its saturated solution. Filter with a vertical molten glass funnel.
Chloraldehyde hydrate test solution Take 50g of chloral hydrate, add 15ml of water and 10ml of glycerin to dissolve, that is, get.
Ferric salicylate test solution (1) Take 0.1g of ferric ammonium sulfate, add 2ml of dilute sulfuric acid and appropriate amount of water to make 100ml. (2) Take 1.15g of sodium salicylate and add water to dissolve it into 100ml. (3) Take 13.6g of sodium acetate and add water to dissolve it into 100ml. (4) Take 1ml of the above ferric ammonium sulfate solution, 0.5ml of sodium salicylate solution, 0.8ml of sodium acetate solution and 0.2ml of dilute acetic acid, mix before use, add water to make 5ml, shake well, that's it.
Glycerin Acetic Acid Test Solution Take 1 part each of glycerin, 50% acetic acid and water, and mix. "Formaldehyde solution" can be used for formaldehyde test solution. Take 1ml of sulfuric acid in formaldehyde sulfuric acid test solution, add 1 drop of formaldehyde test solution, shake well, and you get it. This solution should be used in a new system.
P-dimethylaminobenzaldehyde test solution Take p-dimethylaminobenzaldehyde 0.125g, add a cold mixture of 65ml of nitrogen-free sulfuric acid and 35ml of water to dissolve, add 0.05ml of ferric chloride test solution, shake well. This solution is not applicable 7 days after preparation.
P-toluenesulfonyl-L-arginine methyl ester hydrochloride test solution Take 98.5 mg of p-toluenesulfonyl-L-arginine methyl ester hydrochloride and add trishydroxymethylaminomethane buffer (pH8.1) Dissolve 5ml, add indicator solution (take an equal amount of 0.1% methyl red ethanol solution and 0.05% methylene blue ethanol solution, mix) 0.25ml, dilute to 25ml with water.
P-aminobenzenesulfonic acid-α-naphthylamine test solution: Take 0.5 g of anhydrous p-aminobenzenesulfonic acid, add 150ml of acetic acid to dissolve, then take 0.1g of hydrochloric acid-α-naphthylamine, add 150ml of acetic acid to dissolve, and mix the two , That's it. This liquid is pink for a long time, and zinc powder can be used for decolorization.
Take 1.5 g of p-hydroxybiphenyl test solution, add 10 ml of 5% sodium hydroxide solution and a small amount of water to dissolve, then add water to dilute to 100 ml. This solution is stored in a brown bottle and can be kept for several months.
Potassium ferrocyanide test solution: Take 1g of potassium ferrocyanide and add 10ml of water to dissolve it. This solution should be used in a new system.
Sodium bisulfite test solution: Take 10g of sodium bisulfite, add water to dissolve it into 30ml, that is, get it. This solution should be used in a new system. Sodium sulfite test solution: Take 20g of anhydrous sodium sulfite and add 100ml of water to dissolve it. This solution should be used in a new system.
Sodium nitroferrocyanide test solution Take 1g of sodium nitroferrocyanide and add water to dissolve it into 20ml. This solution should be used in a new system.
Sodium nitroferrocyanide acetaldehyde test solution: take 10ml of 1% sodium nitroferrocyanide solution, add 1ml of acetaldehyde, and mix well. Sodium nitrite test solution: Take 1g of sodium nitrite, add water to dissolve it into 100ml, then you get it.
Take 10g of sodium cobalt nitrite test solution, add water to dissolve it into 50ml, and filter it. Hemoglobin test Take 1g of bovine hemoglobin, add hydrochloric acid solution (take 1mol / L hydrochloric acid solution 65ml, add water to 1000ml) to dissolve into 100ml, that is, get. Store this liquid in the refrigerator for use within 2 days.
Take hydrogen peroxide solution (30%) and dilute it with water to make a 3% solution.
Sodium hypochlorite test solution: take 20g of chlorinated lime, slowly add 100ml of water, grind to a uniform suspension, add 100ml of 14% sodium carbonate solution, stir with the addition, filter with wet filter paper, divide 5ml of the filtrate, add sodium carbonate A few drops of the test solution, if it is turbid, then add an appropriate amount of sodium carbonate solution to completely precipitate the lime and filter it. Contain NaClO should not be less than 4%. This solution should be placed in a brown bottle and kept in a dark place.
Use sodium hypobromite test solution to take 20g of sodium hydroxide, add 75ml of water to dissolve, add 5ml of bromine, and then add water to dilute to 100ml. This solution should be used in a new system.
Take isoniazid test solution 0.25g isoniazid, add hydrochloric acid 0.31ml, add methanol or absolute ethanol to dissolve into 500ml, that is, get.
Ammonium polysulfide test solution Take ammonium sulfide test solution and add sulfur to make it saturated.
Take 1.0g of phthalaldehyde test solution, add 5ml of methanol and 0.4mol / L boric acid solution (adjust pH to 10.4 with 45% sodium hydroxide solution) 95ml, shake to dissolve phthalaldehyde, add Thiol glycolic acid 2ml, adjust the pH value to 10.4 with 45% sodium hydroxide solution.
Take copper tartarate test solution containing iodine, copper sulfate 7.5g, potassium sodium tartrate 25g, anhydrous sodium carbonate 25g, sodium bicarbonate 20g and potassium iodide 5g, dissolve in 800ml water in turn; take another potassium iodate 0.535g, dissolve in water Slowly add to the above solution, then add water to make 1000ml, that's it.
Take resorcinol test solution 1g resorcinol, add hydrochloric acid to dissolve into 100ml, that is. In the test solution of phloroglucinol, take 0.5g of phloroglucinol and add ethanol to dissolve it into 25ml. This solution should be placed in a glass stopper and kept in a dark place.
Take 3g of freshly distilled phenol in the phenol disulfonic acid test solution, add 20ml of sulfuric acid, heat on a water bath for 6 hours, and pour into a glass stopper when it has not yet solidified. When used, it can be placed on a water bath and heated slightly to melt.
Take ninhydrin test solution 2g ninhydrin, add ethanol to dissolve into 100ml, that is.
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