Model prediction, experimental verification and physiological effects of plant membrane transport

The cell membrane is the center that controls cell oscillation, which is very common in the plant kingdom. For example, the periodic change of plasma membrane potential, the fluctuation of vacuolar potential and current, the change of apoplast concentration, the fluctuation of intracellular pH and Ca2 +, the oscillation of plasma membrane transmembrane ion flow of different cell types, etc. However, at present, apart from a general understanding of the direct and indirect involvement of membranes and ion currents in the oscillation process, researchers still know very little about the physiological function of this oscillation. Researchers such as Shabala proposed many ideas using the feedback-controlled oscillation model: (1) The oscillation period strongly depends on the activity of the proton pump; (2) When the activity of the proton pump is suppressed, the oscillation stops; (3) There is a flow between the H + and K + flows Directional change; (4) There are "windows" on the cell membrane that are sensitive to changes in external temperature and ion concentration, and cells exhibit different oscillation modes for different changes in the outside world; (5) Oscillation characteristics are closely related to cell size. They used "non-damage micro-measurement technology" to directly measure the oscillation laws of H +, K +, Ca2 + and O2 across plant membranes, roots, leaves and fungal cells under different environmental conditions. Through the comparison of model prediction and experimental measurement data, the experiment has successfully verified the correctness of the previous assumption. This study combined with model prediction and experimental data, based on the plant's adaptive response to salt, temperature, osmosis, hypoxia and pH stress, made a clearer explanation of the physiological function of oscillation. This model can guide the selection of breeding work on salt resistance and flood resistance. Keywords: Adaptation; Ion flux; Membrane Reference: Shabala S et al. J. Exp. Bot. 2006, 57: 171-184.

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