Early summer is coming, pay attention to health, and now purchase the second box of Shanghai Hengyuan ELISA kit at half price, and send a box of fresh pomegranate. You can also enjoy our company's free testing and technical guidance, authentic agents, and full warranty products. You are welcome to call Mr. He to negotiate with him.
It uses qualitative sandwich immunoassay technology and coats microplate strips with synthetic HEV polypeptide antigens. These peptides are peptides with strong antigenicity in the core amino acid sequence of Chinese HEV strains, which are derived from the strain's Open reading frame 2 and open reading frame 3. Add the sample or standard to the well and incubate. If there is HEV IgM antibody, these antibodies will bind to the HEV polypeptide antigen and be fixed on it. Wash the plate to remove other non-specific antibodies and other components in the sample.
Then add goat anti-human IgM-HRP (horseradish peroxidase) enzyme conjugate. After the second incubation, the enzyme conjugate will be combined with the HEV IgM antibody bound in the first incubation. For the combined enzyme conjugate, add TMB substrate solution. During the third incubation, an enzyme-substrate reaction will occur. Only those wells containing the complex formed by the HEV IgM antibody and enzyme conjugate will change color. Add The sulfuric acid solution stops the reaction between the enzyme and the substrate, and the OD value is measured at the wavelength: 450 nm. According to the test standard of this HEV IgM antibody elisa kit, samples with an OD value greater than or equal to the Cut-Off value are considered positive for the initial test.
The latest American RD, TSZ brand pig ELISA kit:
1 Endothelin 1 7 Somatotropin-releasing hormone 2 Immunoglobulin M (IgM) 8 Follicle stimulating hormone 3 Immunoglobulin G (IgG) 9 Epidermal growth factor 4 Macrophage inflammatory protein 1α 10 Porcine interleukin 6 (IL-6) 5 Matrix metalloproteinase inhibitor 11 interleukin 1 (IL-1) 6 interferon gamma 12 type III procollagen peptideNeed to avoid problems when adding samples in the experiment:
Generally there are 3 sample addition steps: adding specimen, adding enzyme conjugate, and adding substrate. After the frozen serum is thawed, the protein is locally concentrated and unevenly distributed. It should be mixed well and gently to avoid air bubbles. It can be mixed upside down and not vigorously shaken on the mixer. The preparation of plasma specimens requires the help of anticoagulants, while serum specimens can be obtained only after the serum naturally coagulates and the blood clots contract. You can also add diluent to the wells of the plate, and then add serum samples to it, and then shake on the micro-shaker for 1 minute to ensure mixing. Generally speaking, serum specimens measured within 5 days can be placed at 4 ° C, and cryopreservation should be kept at low temperature for more than one week.
Our company mainly acts as an agent for imported ELISA kits imported from well-known American brands. The advantages are advanced, perfect and stable. You are welcome to call our manager He.
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