Application of Chlorophyll Fluorometer in the Study of Stress of Constant and Trace Elements

Plant growth requires both macroelements and microelements. Studying various nutrient stresses is of great significance for plant physiology. A large number of studies have shown that fluorescence parameters such as Fv / Fm, Yield, and ETR have a significant correlation with CO2 fixation, so these fluorescence parameters are widely used in nutrient stress studies.

However, these fluorescence parameters have limitations in characterizing certain nutrient stresses, some can only characterize early stress, some can only characterize moderate stress, and some can only characterize severe stress. Normally, the level of nutrient stress does not affect PSII, and can only be found when it reaches moderate or severe stress.

Therefore, a large number of fluorescence and light absorption tests are applied to the study of different types of nutrient stress, and a variety of different tests are available for researchers to study different nutrient stresses.

This article focuses on the applicability and limitations of common fluorescence parameters Fv / Fm, Yield, etc. in characterizing plant nutrient stress.

Fv / Fm——Dark adaptation measurement

1. Fv / Fm is sensitive to N stress only when the nitrogen level is very low (Baker 2004)

2. Fv / Fm is only sensitive to S stress when the sulfur element reaches a severe stress level (Baker 2004)

3. Fv / Fm is insensitive to nickel (Ni) stress (Joshi 2004)

4. Fv / Fm is insensitive to zinc (Zn) stress (Joshi 2004)

Yield or F / Fm '-measurement under light conditions

1. Yield is insensitive to S stress, unless severe stress is achieved (Baker 2004)

Remarks

Fv / Fm measurement requires dark adaptation. OS-5P, OS-30P and other fluorometers produced by OSI can achieve dark adaptation measurement.

Yield parameters need to be measured when photosynthesis reaches steady state under light conditions, and dark adaptation is not required.

Yield, K-Step, Performance Index, NPQ, qP, FRFex360 / FRFex400 (ratios of the far-red fluorescence intensities induced by 360 nm- and 440 nm-laser pulses) and other parameters are only available for OS-5P. The light absorption under the stress condition of N element and S element can be completed with a chlorophyll relative content analyzer such as CCM-200.

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The following table shows the research results of the effects of constant and trace element nutrient stress on common fluorescence parameters

nutrient

Measurement parameters

references

Fv / Fm

Yield

B (boron)

Yield and ETR

Kshtori R., 1995

unknown

Y

Ca (Calcium)

Fv / Fm

Shmidts-Eiberger 2002

Y

unknown

Cl (chlorine)

No report

——

——

——

Co (cobalt)

Yield

Joshi 2004

Tripaathy 1983

unknown

Y

Cu (copper)

1. Yield

2. Fo / F 5min

1. Joshi 2004

2. Adams2000, Kriedemann1985

unknown

Y

Fe (iron)

OJIP

Jiang 2006

unknown

unknown

Mn (manganese)

OJIP

Hermans 2006

unknown

unknown

Mo (Molybdenum)

No report

——

——

——

Ni (nickel)

ETR

Joshi 2004

Tripathy 1981

N

may

N (nitrogen)

1. FRFex / FRFex440

2. Yield when the light intensity is large

3. K-step

4. Chlorophyll measurement

5. Yield

6. qP

1. Sampson 2000

2. Cheng 2001

3. Strasser 2004

4. Peterson 2006

5. Cavender-Bares 2004, Barker 2004

6. Baker 2004

Only under severe coercion

Barker 2004

Better when the light intensity is large

Cheng 2001

P (phosphorus)

1. PI coefficient in OJIP

2. Fv / Fm

1. Ripley 2004

2. Stark 2000

Y

unknown

K (potassium)

1. Yield

2. NPQ

3. qP

1. Weng 2008

2. Weng 2008

3. Weng 2008

unknown

Y

S (sulfur)

Chlorophyll measurement

Peterson 2006

Only when severe coercion

Barker 2006

Only when severe coercion

Braker 2006

Zn (zinc)

Fs in Yield

Joshi 2004

Tripathy 1980

N

Correlation is lower than Fs